pure ftpd
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pure-ftpd安装
-- phpMyAdmin SQL Dump -- version 3.4.8
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Microarray analysis unmasked two siblings with pure hereditary spastic paraplegia shared a run of
Microarray analysis unmasked two siblings with pure hereditary spastic paraplegia shared a run of homozygosity region onchromosome 3q28–q29
JournaloftheNeurologicalSciences359(2015)351–355
ContentslistsavailableatScienceDirect
JournaloftheNeurologicalSciences
journalhomepage:/locate/jns
Microarrayanalysisunmaskedtwosiblingswithpurehereditaryspasticparaplegiasharedarunofhomozygosityregiononchromosome3q28–q29
WenqianYua,1,XiangdongYoub,1,DongWanga,KaiDonga,JingSuc,ChuanfenLic,JinxiuLiua,QianqianZhanga,FengYoua,XiangrongWanga,JingHu
D6492 Cycle Pure Kit 中文说明书
D6492 Cycle Pure Kit 中文说明书
1、将PCR产物转移到新的1.5mL离心管中,加入4-5倍体积的Buffer CP. 若PCR产物< 200 bp,则加入 6倍体积 Buffer CP。
2、涡旋振荡混匀,瞬时离心收集全部混合液。
3、将上述混合液全部转移到HiBind® DNA吸附柱中,10000rpm室温离心1min,弃废液。
4、向HiBind® DNA吸附柱中加入700 µl的DNA Wash Buffer,10000rpm室温离心1min,弃废液。
5、重复步骤5。
6、13300rpm室温离心2min,甩干HiBind® DNA吸附柱的结合膜。
7、将HiBind® DNA吸附柱套入新的1.5mL离心管中,向HiBind® DNA吸附柱的结合膜正中央加入15-30µl的Elution Buffer,室温放置1-2min,13300rpm室温离心1min。
8、将离心管中的洗脱液重复加入HiBind® DNA吸附柱的结合膜正中央,室温放置1-2min,13300rpm室温离心2min。
Microarray analysis unmasked two siblings with pure hereditary spastic paraplegia shared a run of
Microarray analysis unmasked two siblings with pure hereditary spastic paraplegia shared a run of homozygosity region onchromosome 3q28–q29
JournaloftheNeurologicalSciences359(2015)351–355
ContentslistsavailableatScienceDirect
JournaloftheNeurologicalSciences
journalhomepage:/locate/jns
Microarrayanalysisunmaskedtwosiblingswithpurehereditaryspasticparaplegiasharedarunofhomozygosityregiononchromosome3q28–q29
WenqianYua,1,XiangdongYoub,1,DongWanga,KaiDonga,JingSuc,ChuanfenLic,JinxiuLiua,QianqianZhanga,FengYoua,XiangrongWanga,JingHu