isolation
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Scalable End-to-end Multicast Tree Fault Isolation
Abstract. We present a novel protocol, M3L, for multicast tree fault isolation based purely upon end-to-end information. Here, a fault is a link with a loss rate exceeding a specified threshold. Each receiver collects a trace of end-to-end loss measurement
ScalableEnd-to-endMulticastTreeFaultIsolation
TimurFriedman1,DonTowsley2,andJimKurose2
Universit´ePierreetMarieCurie
LaboratoireLiP6-CNRS
8rueduCapitaineScott
75015Paris,France
timur.friedman@lip6.fr
2UniversityofMassachusettsAmherst
DepartmentofComputerScience
140GovernorsDri
Isolation and functional identification of a novel human hepatic growth factorhepatopoietin Cn
LIVER INJURY/REGENERATION Isolation and Functional Identi?cation of a Novel Human Hepatic Growth Factor:Hepatopoietin Cn Chun-Ping Cui,1*Da-Jin Zhang,1*Bing-Xing Shi,1Shao-Jun Du,1Dan-Li Wu,1Ping Wei,1Gen-Shen Zhong,1
Zi-Kuan Guo,1Yang Liu,2Li-Sheng Wang,1and Chu-Tse Wu1
Hepatic stimulating substance(HSS)was?rst isolated from weanling rat liver in1975and found
to stimulate hepatic DNA synthesis both in vitro and in vivo.Since then,mammalian and human
HSS have been investigated for their potential to treat hepatic diseases.Howev
Design of unequal Wilkinson power divider for dual-band operation with isolation stubs
功分器资料
DesignofunequalWilkinsonpowerdividerfordual-bandoperationwithisolationstubs
X.Li,Y.-J.Yang,L.Yang,S.-X.Gong,T.Hong,X.ChenandY.-J.Zhang
ThedesignofanovelunequalWilkinsonpowerdividerfordual-bandoperationispresented.Theisolationstubsareintroducedtoreducetheparasiticeffectsbetweenthetwostubsthatareconnectedbytheiso-lationresistor.Closed-formdesignequationsarederivedbasedonnetworktheory.Thevalidityofthisanalysisiscon rmedthroughthedesign,simulationandexperimentalresultsforapowerdividerfor1and2.5GHz.
Introduction:Powerdividersa
mirvanaTM mirna isolation kit AM1560 中文说用说明翻译
mirvana mirna isolation kit AM1560
1.贴壁细胞可以直接用PBS在培养板里洗涤
2.后直接加入300-600ul的Lysis/Binding Buffer 然后用细胞刮刮起放在1.5ml的离心管里，剧烈振荡以破解细胞释放miRNA
3.加入1/10 体积的 miRNA Homogenate Additive蜗旋后在冰上放置10min.
4.加入和Lysis/Binding Buffer等体积苯酚和氯仿,蜗旋混匀30-60s后,最大9000g离心.
5.取水相(上层)到一个新的管子里. 预热洗涤液到95度 100%酒精必须室温
6.Add 1/3 volume 100% ethanol, and mix thoroughly 加入1/3体积的100%酒精到水相里蜗旋充分混匀.
7.把液体加到柱子里15s, 9000g离心(9000rpm),超过时间或速度会引起管子损坏
8收集滤液后添加2/3体积的100%室温酒精(如400ul 添加266ul)充分混匀
9.混合物第二次加入一个新的柱子离心15s, 9000rpm,后弃去滤液. 10 用700ul的miRNA Wash Solution 1瞬时离心5-10s 1
isolation of LPMC 小鼠肠粘膜固有层单个核细胞的分离和纯化
小鼠肠粘膜单个核细胞的分离和纯化 一．材料： （一）试剂：
1×Phosphate-buffered saline (PBS)、1×HBSS、FBS、100 U/ ml青霉素, 100 U/ ml链霉素、细胞分离液（Percoll） （二）器材：
剪刀、镊子、培养皿、40um和100um的细胞筛、15ml和50ml离心管、巴氏管 （三）试剂配制：
1）预消化液--HBSS (Hanks Balanced Salt Solutions)：5mM EDTA (100*)和1mM DTT(2000*)。
2）消化液：将0.05 g collagenase D (Roche)、0.05 g DNase I (Sigma)和0.3 g dispase II (Roche)溶解于100ml 1*PBS中。临用临配，配制后37℃孵育几分钟。 3）FACS缓冲液：1*PBS+3% FBS (vol/vol)。
4）40% Percoll(vol/vol)(100mL)：42.01 ml Percoll分离液（Sigma，密度为 1.124 g/ml）+57.99ml 1*PBS。
5）80% Percoll(vol/vol