pFastBac-GST-N使用说明 - 图文

pFastBac-GST-N编号

北京华越洋VECT75436

pFastBac-GST-N载体基本信息：启动子:复制子:终止子:质粒分类:质粒大小:质粒标签:原核抗性:筛选标记:克隆菌株:培养条件:表达宿主:诱导方式:5'测序引物:3'测序引物:备注:PolyhedrinpUCori，F1oriSV40poly(A)signal昆虫细胞载体；杆状病毒组成型表达质粒5463bpC-3C，C-GST氨苄青霉素Ampicillin庆大霉素GentamicinDH5α37℃，有氧，LB昆虫细胞组成型表达，无需诱导pFastbac-F:TATTCCGGATTATTCATACCpFastBac-R:ACAAATGTGGTATGGCTGA可表达GST融合蛋白载体名称pFastBac-GST-N

pFastBac-GST-N载体图谱：

pFastBac-GST-N载体简介：

TheBac-to-Bac?BaculovirusExpressionSystemprovidesarapidandefficientmethodtogeneraterecombinantbaculoviruses(Ciccaroneetal.,1997).ThismethodwasdevelopedbyresearchersatMonsanto,andisbasedonsite-specifictranspositionofanexpressioncassetteintoabaculovirusshuttlevector(bacmid)propagatedinE.coli(Luckowetal.,1993).ThemajorcomponentsoftheBac-to-Bac?BaculovirusExpressionSysteminclude:

?AchoiceofpFastBac?donorplasmidsthatallow

generationofanexpressionconstructcontainingthegeneofinterestwhereexpressionofthegeneofinterestiscontrolledbyabaculovirus-specificpromoter.

?AnE.colihoststrain,DH10Bac?,thatcontains

abaculovirusshuttlevector(bacmid)andahelperplasmid,andallowsgenerationofarecombinantbacmidfollowingtranspositionofthepFastBacexpressionconstruct.

pFastBac-GST-N载体序列：

ORIGIN1gacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgacc61gctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgcc121acgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgattt181agtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtggg241ccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagt301ggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgattta361taagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaattt421aacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaat481gtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatg541agacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaa601catttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcac661ccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttac721atcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgtttt781ccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgcc841gggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactca901ccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgcc961ataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaag1021gagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaa1081ccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatg1141gcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaa1201ttaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccg1261gctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcatt1321gcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagt1381caggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaag1441cattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcat1501ttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatccct1561taacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttct1621tgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctacca1681gcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttc1741agcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttc1801aagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgct1861gccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataag1921gcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacc1981tacaccgaactgagatacctacagcgtgagcattgagaaagcgccacgcttcccgaaggg2041agaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggag2101cttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgactt2161gagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaac2221gcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcg2281ttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgc2341cgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatg2401cggtattttctccttacgcatctgtgcggtatttcacaccgcagaccagccgcgtaacct2461ggcaaaatcggttacggttgagtaataaatggatgccctgcgtaagcgggtgtgggcgga2521caataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtcttaaactag2581acagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttt2641tgttatggctaaagcaaactcttcattttctgaagtgcaaattgcccgtcgtattaaaga2701ggggcgtggccaagggcatggtaaagactatattcgcggcgttgtgacaatttaccgaac2761aactccgcggccgggaagccgatctcggcttgaacgaattgttaggtggcggtacttggg2821tcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcg2881ggatcgtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctca2941tgcttgaggagattgatgagcgcggtggcaatgccctgcctccggtgctcgccggagact3001gcgagatcatagatatagatctcactacgcggctgctcaaacctgggcagaacgtaagcc3061gcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttacta3121cggagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtct3181ccgaactcacgaccgaaaagatcaagagcagcccgcatggatttgacttggtcagggccg3241agcctacatgtgcgaatgatgcccatacttgagccacctaactttgttttagggcgactg3301ccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatcaaaca3361tcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaa3421acagtcataacaagccatgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaa3481ggttctggaccagttgcgtgagcgcatacgctacttgcattacagtttacgaaccgaaca3541ggcttatgtcaactgggttcgtgccttcatccgtttccacggtgtgcgtcacccggcaac3601cttgggcagcagcgaagtcgaggcatttctgtcctggctggcgaacgagcgcaaggtttc3661ggtctccacgcatcgtcaggcattggcggccttgctgttcttctacggcaaggtgctgtg3721cacggatctgccctggcttcaggagatcggaagacctcggccgtcgcggcgcttgccggt3781ggtgctgaccccggatgaagtggttcgcatcctcggttttctggaaggcgagcatcgttt3841gttcgcccaggactctagctatagttctagtggttggctacgtatactccggaatattaa3901tagatcatggagataattaaaatgataaccatctcgcaaataaataagtattttactgtt3961ttcgtaacagttttgtaataaaaaaacctataaatattccggattattcataccgtccca4021ccatcgggcgcggatcccggtccgaagcgcgcggaattcaaaggcctacgtcgacgagct4081cactagtcgcggccgctttcgaatctagacatatgggtaccctcgagggcagcagcctgg4141aagttctgttccaggggccctcggatatgtcccctatactaggttattggaaaattaagg4201gccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatt4261tgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagt4321ttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatca4381tacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagaga4441tttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatata4501gtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaa4561tgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctg4621acttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatg4681cgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagt4741acttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtg4801gtggcgaccatcctccaaaatgaaagcttgtcgagaagtactagaggatcataatcagcc4861ataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacc4921tgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggtt4981acaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattcta5041gttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatctgatcactgcttg5101agcctaggagatccgaaccagataagtgaaatctagttccaaactattttgtcattttta5161attttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttcccta5221aataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccac5281agcggggcatttttcttcctgttatgtttttaatcaaacatcctgccaactccatgtgac5341aaaccgtcatcttcggctactttttctctgtcacagaatgaaaatttttctgtcatctct5401tcgttattaatgtttgtaattgactgaatatcaacgcttatttgcagcctgaatggcgaa5461tgg//pFastBac-GST-N载体其他相关的昆虫细胞载体：pFastBacHTCpFastBacHTBpFastBacHTA

pFastBacDualpFastBac1pAC5.1b-EGFPpVL1392pAcGP67A

pFastBac-GST-NpFastBac-GST-C

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