OVERVIEW Reagent solutions VPA Combo 260624（阳极溶出伏安法试剂配方）要点

Thursday, 26st June 2013

REAGENT SOLUTIONS

The vessels used for the preparation of the reagents must be as clean as possible, preferably made of Teflon, PE or PP materials. The best is to use the vessels always for storing and preparation of the same solutions, avoiding so cross-contamination.用于制备试剂的容器应该尽可能的干净，最好用聚四氟乙烯，聚乙烯或聚丙烯材料。最好同一个容器装同一个试剂，以避免交叉感染。

If used vessels are applied, clean the vessels 3 times with Potable water, 3 times with 0.01M Nitric acid (HNO3) and 3 times with Demineralized water.如果容器已经使用过，最好用饮用水清洗三次，用0.01M的硝酸2清洗三次，去离子水清洗三次。

The reagents used should be of highest purity regarding the content of measured species. Especially great care is needed if some common elements such as Zn, Pb, Cu, Fe, Mn, etc. have to be measured. Most commercial reagents may contain traces of these elements and may be the reason of deteriorated measurements. Hence, it is advisable to test the used reagents for purity first, the best by ICP-MS or similar sensitive method.所用的试剂应纯度最高的含量测定。如果一些共同的元素如Zn，Pb，Cu，Fe，Mn等需要非常谨慎。大多数商业试剂可能含有痕量的这些元素，并可能是影响测量的原因。因此，检测用试剂的纯度，通过ICP-MS或类似的灵敏的方法最好。

Demineralized water used for preparation should not contain dissolved gases (air) and microbes, so boil the water shortly and cool down to ambient temperature. This degassed Demineralized water will be suitable for reagent preparation. Obviously, clean vessels should be used for boiling配制试剂所用的去离子水应该不含气体或者微生物。所以煮沸的水应该冷却到室温。去除气体的去离子水应该适用于试剂配制。显然，清洗容器也应该用煮沸的水。

Some reagents may contain solid particles which may not dissolve in water. Filter such solutions through a clean paper filter (rinse the filter device and filter paper with diluted Nitric acid and water first).有些试剂应该含有不溶解水的固体颗粒。应该用清洁的滤纸过滤试剂（过滤装置应该用稀硝酸和水先清洗）

Dust-free environment during reagent preparation ensures higher reliability regarding the purity of the reagents.在死机配制过程中无尘环境和试剂的纯度可以确保高可靠性。

? Also refer to the word document ‘RECOMMENDATION - Preparation Reagent solutions 1.1’.

PREPARATION OF CLEAN SOLUTION (0.01M HNO3)制备清洗液 （0.01M HNO3）

Ingredients成分:

? Water: demineralized water boiled and cooled down before use, 水：在使用前煮沸的去离子水冷却。

? Nitric acid 65% (HNO3) GR for analysis (max. 0.005 ppm Hg) (VWR/Merck). 65%的优级纯HNO3用于分析（max.0.005ppm Hg）(VWR/Merk) Preparation of 2 L 0.01M Nitric acid制备2L0.01M硝酸: Dissolve in 1000 mL demineralized water: - 1,43 mL HNO3.

1.43ml的HNO3溶解在1000ml去离子水中。

Adjust the volume to 2 L and mix the solution.

调整体积到2L并且混合溶液

?Notes注意: Prepare the reagent solution in a reliable fume hood!在一个可靠的通风柜准备试剂溶液 Use security glasses and gloves!使用安全眼镜和手套

?REFERENCES REAGENTS参考试剂

? Nitric acid solution 65% GR for analysis (max. 0.005 ppm Hg) 65%的优级纯HNO3用于分析（max.0.005ppm Hg）

MSDS 100452_EN_EU.pdf HNO3

GR for analysis (max. 0.005 ppm Hg), ISO

3Mm 63.01 g/mol, d 1.41 g/cm VWR no. 1.00452

PREPARATION OF DILUTION SOLUTION (0.01M HCl)制备稀释液（0.01M HCl）

For preparation of Calibration solutions and Test Solutions Pb, Cd, Cu, Fe, Ni and Zn. 制备校准试剂个测试Pb, Cd, Cu, Fe, Ni and Zn. Ingredients成份: ? Water: demineralized water boiled and cooled down before use, 水：使用前将煮沸的去离子水并且冷却

37%? Hydrochloric acid 37% (HCl) GR for analysis (max. 0.001 ppm Hg) (VWR/Merck).

的HCl用于分析（max.0.001ppm Hg）(VWR/Merk).

Preparation of 2 L 0.01M Hydrochloric acid: 配制2L0.01M的HCl溶液：

Dissolve in 1000 mL demineralized water:

- 1,67 mL HCl.取1.67mlHCl溶解到1000ml去离子水中

Adjust the volume to 2 L and mix the solution.调整体积到2L并且混合溶液

?Notes:

Prepare the reagent solution in a reliable fume hood!Use security glasses and gloves!使用安全眼镜和手套在一个可靠的通风柜准备试剂溶液

?REFERENCES REAGENTS参考试剂

?

Hydrochloric acid Fuming 37% GR for analysis (max. 0.001 ppm Hg) MSDS 113386_EN_EU.pdf HCl

37% GR for analysis (max. 0.001 ppm Hg)

Mm 36.46 g/mol, d 1.19 g/cm3 VWR no. 1.13386

PREPARATION OF THE R-315 REAGENT配制R-315试剂

Ingredients成分:

Water: demineralized water boiled and cooled down before use, 水：使用前将煮沸的去离子水并且冷却

Hydrochloric acid 37% (HCl) GR for analysis (max. 0.001 ppm Hg) (VWR/Merck). 37%的HCl用于分析（max.0.001ppm Hg）(VWR/Merk).??? ?

Triton X-100, GR for analysis (VWR/Merck),去拉通 x-100 Sodium chloride (NaCl) (VWR/Merck),氯化钠（VWR/MercK）

Preparation of 5 L reagent solution R-315:配制5L试剂溶液R-315

-

Add 29.3 g of NaCl and 0.85 mL of concentrated hydrochloric acid ( or 10 mL of 1.0 mol/L HCl) to

1 L of water in a 5 L vessel,溶解29.3gNaCl和0.85ml浓盐酸（或者10ml 1.0mol/L HCl）至1L

水中在一个5L的容器中。

- -

Adjust the volume to 5 L with water,用水调整体积到5L

Add 1.0 mL of Triton and mix the solution.加入1.0ml去拉通并且混合溶液

After 1-2 hours mix the solution again and now it can be used.1-2小时以后再次混合溶液并且现在可以使用了。

?Shelf life（保质期）: 1 year.1年。 ?REFERENCES REAGENTS

? Hydrochloric acid Fuming 37% GR for analysis (max. 0.001 ppm Hg)

MSDS 113386_EN_EU.pdf HCl

37% GR for analysis (max. 0.001 ppm Hg)

3Mm 36.46 g/mol, d 1.19 g/cm VWR no. 1.13386

?

? TritonX-100

C8H17C6H4(OCH2CH2)nOH GR pro analysi Ph Eur d 1.07 g/cm3

CAS no. 9002-93-1 VWR no. 1.08643 ? Sodium chloride

MSDS 106404_EN_EU.pdf NaCl

GR for analysis, ACS, ISO Mm 58.44 g/mol, d 2.17 g/cm3 CAS no. 7647-14-5 EC no. 231-598-3 VWR no. 1.06404

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