南瓜花黄酮超声波辅助提取工艺的研究

南瓜花黄酮超声波辅助提取工艺的研究

摘要:在单因素试验的基础上采用L9(34)正交试验设计,考察了料液比?提取时间?乙醇浓度?提取温度对总黄酮提取率的影响,以确定南瓜花中总黄酮的优选超声波辅助提取工艺?结果显示,提取温度40℃?料液比1∶18(m/V,g∶mL)?乙醇浓度40%?提取时间40 min,提取效果较佳,总黄酮平均提取率可达2.740%?

关键词:南瓜花;总黄酮;超声波;提取工艺

Study on Ultrasonic-assisted Extration Process of Total Flavonoids in Pumpkin Flower

Abstract: To optimize the ultrasonic-assisted extraction process of total flavonoids from pumpkin flower, the effect factors including solid-liquid ratio, extraction time, solvent concentration and extraction temperature were studied through orthogonal tests L9(34) on the basis of single factor test. The total flavonoids in pumpkin flower was determined through spectrophotometry by using rutin as standard sample. The optimum conditions to extract total flavones was as follows: extraction temperature,40℃; solid-liquid ratio, 1∶18(m/V,g∶mL); ethanol concentration,40%; extraction time, 40 minutes; the extraction rate of total flavonoids reached 2.740% under these conditions

Key words: pumpkin flower;total flavonoids;ultrasonic-assisted technology;extraction process

花是植物的精华,现代科学证明,不少花卉含丰富的营养成分,有强身健体的作用[1,2]?南瓜

[Cucurbita moschata(Duch.)Poiret]的花朝开暮合,灿若金星,戴露迎霜,南方一些地方常将其调制成餐桌上的一道道美味佳肴?南瓜花亦蔬亦药,具清利湿热?消肿散瘀?防癌抗癌作用,适于治疗黄疸?痢疾?咳嗽?痈疽及结膜炎?乳腺炎等诸多炎症,常作强身保健食品?实践证明,其花粉能消除疲劳?增强斗志,对幼儿贫血?慢性便秘?大肠疾患?高血压?头痛?中风等病症有一定的疗效,又能调整神经状态,改善失眠[3]?研究发现,南瓜花的黄酮类化合物含量丰富,可达3.04%[4],被认为是黄瓜花诸多功能的活性成分,因此,对黄瓜花黄酮类化合物的研究很有价值?

近年来,超声技术应用于提取植物中的生物碱?甙类?黄酮类活性物质,动物组织浆的毒质等研究已有报道[5-8]?该技术具有能耗低?效率高?不破坏有效成分的特点?目前南瓜花中黄酮类化合物的提取工艺研究尚未见报道?本试验以南瓜花为对象,采用单因素试验及正交试验相结合的方法,考察超声波辅助提取南瓜花总黄

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