lipofectamineLTX_and_PLUS_man

Lipofectamine®

LTX & Plus Reagent

*Plus Reagent (Cat. no. 11514-015) available separately.

Pub. Part No. 15338.pps

Pub. No. MAN0001225

Rev. Date 20 July 2012

Description

Lipofectamine® LTX and Plus Reagent are proprietary formulations for

transfecting nucleic acids into a wide range of eukaryotic cells. Nucleic acid-Lipofectamine® LTX complexes can be added directly to

cells in culture medium, in the presence or absence of serum/antibiotic.

It is not necessary to remove complexes or change/add medium after transfection.

Important Guidelines for Transfection

Use Opti-MEM® I Reduced Serum Medium (Cat. no. 31985-062) to

dilute Lipofectamine® LTX Reagent and nucleic acids.

The amount of Lipofectamine® LTX Reagent required for successful transfection varies depending on the cell type and passage number. Start any new transfection by testing the recommended four concentrations of Lipofectamine® LTX Reagent to determine an optimum amount.

For additional information, and protocols on transfecting suspension cells refer to the manual at /transfection

For research use only. Not for human or animal therapeutic or diagnostic use.

Cells

yaDLipofectamine® LTX Reagent

DNA and Plus Reagent

1 yaD

4–2 yaD

Plasmid Transfection

Transfect cells according to the following table. The transfection is designed for 1 DNA and PLUS reagent amount combined with 4 different amounts of Lipofectamine® LTX. For each lipid reagent amount, the prepared mix is enough to have triplicates (96-well), duplicates (24-well), and single well

(6-well) transfections, and account for pipetting variations.

The following table shows the amounts of DNA and Lipofectamine® LTX Reagent per well used in each transfection reaction. For additional information on scaling your transfection reaction, see page 4.

Scaling Up or Down Transfections

Use the following table to scale the volumes for your transfection experiment.

determine the reagent amount needed for your new plate format.

2

Optimum amount needed is determined from the protocol (see pages 2–3).

mRNA Transfection

mRNA can be transfected in a 24-well plate by using Lipofectamine® LTX Reagent by adding 0.5–1 μg of mRNA per well. The mRNA can be prepared using mMessage mMachine® SP6 Kit (Cat. no. AM1340).

Limited Product Warranty

Life Technologies Corporation and/or its af liate(s) warrant their products as set forth in the Life Technologies’ General Terms and Conditions of Sale found on Life Technologies’ website at /termsandconditions. If you have any questions, please contact Life Technologies at /support.

Disclaimer: LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR

IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.

©2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.

For support visit /support

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