以endo-beta-1,4-galactanase为例介

以endo-beta-1,4-galactanase为例介绍其氨基酸序列调取、序列比对及建树的流程 一：序列序列调取

1. 在BRENDA（http://www.brenda-enzymes.org/）搜索酶，如下图：在搜索框内输入

“endo-beta-1,4-galactanase”选择搜索内容，如下图篮框“Enzyme”, 点击“Start search”显示搜索结果。

2. 搜索结果见下图

3. 双击红色方框，显示所搜索的酶的氨基酸序列号

4. 双击上图红色序列号，如Q9X0S8，显示搜索酶的氨基酸序列，如下图：

5. 可以下载单个序列，也可以下载所有的数据中的endo-beta-1,4-galactanase，下载序列，

保存。 二、 序列比对

1.下载到的序列比对，选用比对软件”Clustal X”比对，序列载入

2.序列比对

3.保存

三、构建系统发育树

选用MEGA6 构建系统发育树：

Mega因为操作简单，结果美观，很多研究者选择用它来建树。

(1) 首先用Clustal X进行序列比对，剪切后生成D:\\文老师\\酶\\Bifidobacterium.aln文件；

(2) 打开Mega程序，转化为mega格式并激活目标文件，

(3) File-Convert To MEGA Format- D:\\文老师\\酶\\Bifidobacterium.aln →D:\\文老师\\酶

\\Bifidobacterium. meg，

(4) Phylogeny-Neighbor-Joining(NJ) (5) Protein sequence→OK (6) 开始计算－得到结果；

I8YQW3|arabinogalactan434754 D4WP72|arabinogalactan R6J710|arabinogalactan A7M5U2|arabinogalactan100 I9HZH7|arabinogalactan F7LE03|arabinogalactan10057 A0A078PYK0|arabinogalactan R6E3H2|arabinogalactan98100 R5V2B1|arabinogalactan99 I9Q1U4|arabinogalactan92 A5ZGS0|arabinogalactan69 K5CSY8|arabinogalactan100 R6S5B4|arabinogalactan1001007899 C9L001|arabinogalactan R6V9P5|arabinogalactan D7K7G3|arabinogalactan R5VE68|arabinogalactan100 E2NMA6|arabinogalactan R6KPQ5|arabinogalactan909993 A0A069D6L5|arabinogalactan U6RAT1|arabinogalactan61 R7KK27|arabinogalactan Q89YR3|arabinogalactan100 D7IA61|arabinogalactan56 C6IQ23|arabinogalactan6885 R9H881|arabinogalactan100 A0A078QA81|arabinogalactan26 A0A078Q7X7|arabinogalactan R6ZSY5|arabinogalactan100 R5W7T5|arabinogalactan63 A0A078RXS1|arabinogalactan93 I8ZPX3|arabinogalactan43 R7EBX7|arabinogalactan4110099 A7V0W8|arabinogalactan R9HUC6|arabinogalactan39 E5VAD2|arabinogalactan K9DSN8|arabinogalactan10068100 R5S2G0|arabinogalactan R5C116|arabinogalactan69100 B0NQK4|arabinogalactan S3ZJQ9|arabinogalactan999941 D2ESG2|arabinogalactan E6SQF3|arabinogalactan R5M5U9|arabinogalactan100 R7DUP1|arabinogalactan95 I9QMG7|arabinogalactan100 R5RUK2|arabinogalactan R6E3J0|arabinogalactan5135 C3Q3U6|arabinogalactan I8WE00|arabinogalactan A0A076IYP6|arabinogalactan100 B6VYB2|arabinogalactan I9R4F8|arabinogalactan62 D1K6Z6|arabinogalactan C3RCI9|arabinogalactan A0A078RGT2|arabinogalactan D4V3H4|arabinogalactan63 C6YZ45|arabinogalactan E5UN80|arabinogalactan A0A069SNP0|arabinogalactan R2AUA8|arabinogalactan I0F933|arabinogalactan I8YPK4|arabinogalactan0.2

endo-beta-1,4-galactanase bacteroides

90 Q8X168|arabinogalactan99 G3YEH1|arabinogalactan7410094 A2RB93|arabinogalactan G7XSY2|arabinogalactan Q9Y7F8|arabinogalactan P48842|arabinogalactan55100 A2R8B9|arabinogalactan A0A017ST38|arabinogalactan943154 A0A084BVX8|arabinogalactan A0A0F8V5M2|arabinogalactan Q0CTQ7|arabinogalactan A0A0F0ICL5|arabinogalactan47 Q2UN61|arabinogalactan100 A0A0D9N2B1|arabinogalactan99 B8NNI2|arabinogalactan59 I8I846|arabinogalactan73 A0A064B8R8|arabinogalactan A0A0D9N7P5|arabinogalactan100 B8NJE6|arabinogalactan0.05endo-beta-1,4-galactanase aspergillus

I3BMH6|arabinogalactan I3AR57|arabinogalactan D6ZY99|arabinogalactan T2I1T1|arabinogalactan E5XWR1|arabinogalactan37 R5MUT3|arabinogalactan A0A083XX36|arabinogalactan D6D9W5|arabinogalactan50 B3DTI0|arabinogalactan E8MRV4|arabinogalactan100 Q8G7K7|arabinogalactan A0A087BM38|arabinogalactan A0A0A0UHY0|arabinogalactan W6F0I6|arabinogalactan F6C7Q8|arabinogalactan884142 S3AK82|arabinogalactan V6XZF6|arabinogalactan C1J0P1|arabinogalactan89 U2CV73|arabinogalactan0.002

endo-beta-1,4-galactanase Bifidobacterium

8150 Q76FP5|galactan Q7S2S4|galactan K4R0H9|galactan A0A0F8AZ05|galactan A0A086YZJ7|galactan0.1endo-1,6-beta-galactosidase

S1|arabinogalactan46 A0A078RX7349999930 I8ZPX3|arabinogalactan R7EBX7|arabinogalactan R9HUC6|arabinogalactan A7V0W8|arabinogalactan E5VAD2|arabinogalactan F3PQC3|arabinogalactan3834100 K9DSN8|arabinogalactan B0NQK4|arabinogalactan S3ZJQ9|arabinogalactan509678591009731100 E6SQF3|arabinogalactan D2ESG2|arabinogalactan R5M5U9|arabinogalactan R7DUP1|arabinogalactan I9QMG7|arabinogalactan R5S2G0|arabinogalactan R5C116|arabinogalactan100 A0A078QA81|arabinogalactan A0A078Q7X7|arabinogalactan2334100 R6ZSY5|arabinogalactan R5W7T5|arabinogalactan100 R5RUK2|arabinogalactan R6E3J0|arabinogalactan6510089 C3Q3U6|arabinogalactan D4V3H4|arabinogalactan I8WE00|arabinogalactan A0A069SNP0|arabinogalactan65 E5UN80|arabinogalactan69 C6YZ45|arabinogalactan A0A078RGT2|arabinogalactan9940 B6VYB2|arabinogalactan C3RCI9|arabinogalactan44 D1K6Z6|arabinogalactan I9R4F8|arabinogalactan A0A076IYP6|arabinogalactan58 C6IQ23|arabinogalactan53 R9H881|arabinogalactan33100 D7IA61|arabinogalactan R7KK27|arabinogalactan Q89YR3|arabinogalactan1008399 E2NMA6|arabinogalactan R6KPQ5|arabinogalactan A0A069D6L5|arabinogalactan88 U6RAT1|arabinogalactan R5VE68|arabinogalactan6010074 R6V9P5|arabinogalactan D7K7G3|arabinogalactan W4V1K0|arabinogalactan9910057100 R6S5B4|arabinogalactan C9L001|arabinogalactan K5CSY8|arabinogalactan6990 I9Q1U4|arabinogalactan9896100 A5ZGS0|arabinogalactan R5V2B1|arabinogalactan R6E3H2|arabinogalactan9810086 A0A078PYK0|arabinogalactan I9HZH7|arabinogalactan F7LE03|arabinogalactan I8YQW3|arabinogalactan D4WP72|arabinogalactan443716 A7M5U2|arabinogalactan61 R6J710|arabinogalactan A0A087A1D4|arabinogalactan5371991007810054 E5C4Z1|arabinogalactan K5CK73|arabinogalactan F7LFM6|arabinogalactan I9HNU1|arabinogalactan R6EBQ0|arabinogalactan D7K5N6|arabinogalactan C3R215|arabinogalactan18009 A0A078Q6S5|arabinogalactan A0A078PNE9|arabinogalactan74 R6JR07|arabinogalactan86 D4WA18|arabinogalactan R2AUA8|arabinogalactan3|arabinogalactan100 D2Q6T1001003552 A0A0C6DTA1|arabinogalactan E0QAW3|arabinogalactan W4NB52|arabinogalactan I8YPK4|arabinogalactan I0F933|arabinogalactan6062 W6F0I6|arabinogalactan79 F6C7Q8|arabinogalactan731007699 S3AK82|arabinogalactan C1J0P1|arabinogalactan V6XZF6|arabinogalactan U2CV73|arabinogalactan100967789 T2I1T1|arabinogalactan D6D9W5|arabinogalactan I3AR57|arabinogalactan A0A0A0UHY0|arabinogalactan A0A083XX36|arabinogalactan R5MUT3|arabinogalactan36 D6ZY99|arabinogalactan E5XWR1|arabinogalactan101023 B3DTI0|arabinogalactan E8MRV4|arabinogalactan Q8G7K7|arabinogalactan15 I3BMH6|arabinogalactan44100 A0A087BM38|arabinogalactan R9H3N8|arabinogalactan A0A076IZ95|arabinogalactan9888 D2ESH0|arabinogalactan R9I2C7|arabinogalactan E2NM97|arabinogalactan5410098 R5MNS1|arabinogalactan D2ESG1|arabinogalactan10059 A0A0D9N7P5|arabinogalactan B8NJE6|arabinogalactan74 I8I846|arabinogalactan57 A0A064B8R8|arabinogalactan48 B8NNI2|arabinogalactan100100100 A0A0D9N2B1|arabinogalactan Q2UN61|arabinogalactan A0A0F0ICL5|arabinogalactan A0A017ST38|arabinogalactan99583285 A0A0F8V5M2|arabinogalactan A0A084BVX8|arabinogalactan Q0CTQ7|arabinogalactan99 P48842|arabinogalactan A2R8B9|arabinogalactan3557 Q9Y7F8|arabinogalactan G7XSY2|arabinogalactan8510086 A2RB93|arabinogalactan Q8X168|arabinogalactan89 G3YEH1|arabinogalactan0.1

Bif+Asp+Bacteriodes endo-beta-1,4-galactanase

ctanlaninogara|aA0bK5CK73LtaAc0a7lr8galactano5nSi6baa|aQMFg7Fro6nib|arabinaa1|ogalacI9HNU1|arE5C4ZtanabinogalactanA0A078PNE9|arabinogalactanR6EBQ0|arabinD7ogaK5lactN6an|arC3abR2inogR615|alaJRaractabn07ino|aA0Agar0D9lacabN7Ptan5|arinoRag9AbH3inogal0AN8aalata07|aractcan6bIZinonB8NA0AB895gaNI2|0DNJE|arlacara9b6|aNabtanin2Brabog1ainoila|anctanogragaalabinlaccotangalI8IAtanac84t0anA6|a06ra4bBi8nogaR8|alactraanbinogalactan|abarinogacaltanA0A087A1D4|arabinogalactanan|arabinogalactA0A078PYK0I8YQWA7M5U2|arabinogalactan3|aR6Jrabinogalactan1D0|47aWP72rabinogaI9clactan|artanHinogalaZHabinn73|arabcta|arogalacF7LE05|arabinogalaabinotanR6V9Pgalacantanalactinogarab1K0|ctanW4Vgalabino|aralactan7G3gaD7Kbinonat|aralacn001gaanC9Lctanotiaclabalaogactan|arogB4inaniS5abgalbraR6a|ar2|nobiY83HraCSR6E|aB1V2K5D4W8A1A0AI8ZPX3|0a7rabRin9ogalactanHSR3UJQnaca|8gCoinraa4|B0NQKZ9bX|arabAianl6Satog7Vraltaac1btanin|a0RacalWgago8|aabralactan5SnoE6SQF3|arabinR7rinoEEa2b5ningGoBVgat0XcalacalacaAlag|7oarnDib|araa|2|raabtantan2arbininD2ESGantaboocalogainogalganibarlaa|gaacn9cnaUt5ctalataR5Malacgotannibnnracta7|aMGalagQ9oIinab|ar1PDUR7A5A0ZGA0R7IA0S058Q9Q78VE1|a7XUQA6ra8|74|b|aar81ararbQaibabiinog|ara89nniongbinooggaalacYRR7ogalalclacta3|KKalaaatntar27R9nantactacaa|nH8biarnnoab81|arC6IabinoggalQ2ainoa3|agalacctarabU6RD7tliaanogacnnAT1IA61|alacttan|ararabinA0Aanogbi069D6L5|nogalaalactanarabctainognR6KPalactQ5|aranabinogalacA0AC6Ytan078E4N|MA6|arabinogaRGZ2lactanaraT2|5aranogbibniogaaC3RClalaccI9|arantatannnnA0A069SaintbaotgNP0|araaacttalncaccabtaainllalanncolgaaalataaccnntaalaggoggalactaaoogoognnnioniaiinbibbbranD1K6Zi|nacta6|aarrabbla6inoagalgraactoPaninb|brYraaaI6|a|26BrY7UV3a6Q00|aB80A0AC|raatn3Na0atnann4|UE5nc8HatctalcEaWaa3laFclnt8nagaI4ValagglacctaR4ooinctoaDaingI9lainalaograbbogbaana|oniabogbiinT5|ar|ara7Y5N8|arbinWara||a5S023aSRZrK3JEUR6K9DQC6|a6RR5P13R1FC5RA0ginotanbctalaaratan|acnlacalinogM2ctaag5alaoggnoarab8VniiFb|inorababra50|ar|a0ICLA0A38|a81XT60FBVNA7SanU001A0842AlactAa0g0QoAAinctanrab7|agalaoQnibtanCT|araQ0galac842abinor8a|49PBA2R8tanogalacbarain|8FG79Y3YEQQH1|ainogalactan8X1Yab68ara2|rbain|aG7XSbiongoaA2RB93|arrabglaclainoagactanlatanctancalatanR5anR2AUA8|arabinogalactananalactbinogara|3QAWE0A0A0C6inogalactanb1DraTAW4NB52|a|arabinD2Q6T3|arabinoognalacgalactatanI0F933|arabinogalactannlactainogabIa3rDaA|D6F6ZAD9R0657inogalactanV6XAZW0YA90ra5b|a|aU39a0TA|YH0|MAU5ar|a083Rr6XXa3babaain|ainbiorbiirngnonoagglaoE5XWR1|arabinogoalcaalactangatannlagaclatanctalatccatannlactangainooabS3|arabin82|arC1J0P1AKtangalactangalacabinotanQ8|arBalacn3gDaTF6C7tI0|arabbinolacinogalan|aragactanctanRV4inoltaaaU2CE8M|arabgV73acK7ino|alarabinG7rabogogaQ8|ainI3BlactaM0I6abnAH06Far6A|aWT1|0ratan8bin7binogalacI1BoMgaI8YPK4|araT23act8|laaranbinogalactaninrabboraC7|aE2NM97|aR9I2tangalactanalacinoaggabinolactaH0|arRn5MNS1|arabinogalactanD2ESD2ESG1|arabinogalactan0.2AAA00640760A0QBD9NIZ9D9N2U8R5|a2B78|abiBP5|a1N61Br8Ia8R9H|anoIrN8a8|arabragalb4JEN3N8ibria6|inoNIactn6|ao|ara2|aanagoigalanbrabinonocalgalarabrabtgalabinanoinogngaactctactanogialagalalaacnncatalanctactatannnA0A0S3C1AK82|F6J0aPE8C7Q1|arabinMRr8aQ8V4|arabinogaG7ob|lK7arabinogalactW6|arinogaacanF0latabI6|inogalactaanT2ctanI1Tarabgainla1I3AnR5|arabogalactanD6D7|ainoctraga9W5|arbinogalactnabinalaanA0AD6ZYogactan0A0U99|alactHY0rabinan|aorgaA0A0bailnaogalctan83XXacta36|arnabinogalacE5XWtanR1|arabinogalactanR5MUT3|arabinogalactanV6XZF6|arabinogalactanI0F933|arabinogalactanlactanA0A0C6DTA1|arabinogantanacalogino3|arabcttalaaD2Q6T2ganalactanaginbrala|agabiconW4NB53|arabinoaractan8|alctanE0QAWR2AUAgaoactannibrainogallana|1taHrabinogalactanG3YE168|arcbgaaloQ8XB93|arabinoganannabinlactattnaA2RSY2|anraanalcatclta|agaaXccatgG7Y7F8binonoaagoalnlacigianlgQ99|ararabaabtoorainacogin|abBR8427|aabalinraQA24888Tarograb|a|P0C8nVXT3abi5|aQBSr4817|aCLA0A0M2F0I0AA0V50F80AA0A0AR7DUP1|arabinogalactanI9QMG7|arabinogalactanR5M5U9|arabinogalactanD2ESG2|arabinogalactanE6SQF3|arabinogalactanB0NQK4|arabinogalacS3ZtanJQ9|arabI8ZPinogX3|aalactanrabiR9HnogalacUC6tanA0A7A0|arabinV078oWgRXalac8|araStanbi1agn|oraR7bainlaEcotagnalaE5BX7|ctaaraVAnbDR5in2og|arS2aalacG0binRtanogF35C1|araalb1aPinoctaK9Q6|aganDSC3rabla|aricnNtanoa8|arbingalaogabctalinacanogtanalactanctangalabinoan|araactngalSG1aotnD2Elacabirag1|aninoNSctarabR5Malan7|ag9ainolactnNMaE2raba7|abinoglactaI2CaogR9|arnnH0binctanractaESla|aalataD28a3goglacnnoBiMbinogaacta7braraa8al|abinA|004H6rainogPAKaBM3|I8Yab37IarCV0|U2DTIB30.2nanctctanaalacttanglaogacnlnogaiiabgabnorataninoarrabi2|ab|acal5KraatanSYT5|5RUJ0|ainoglacZa3b7g6R6EaoanR5W|arabinactlR8aRg4F4|artaninolacI9RV3Harabago|anbinD4E00lactanaraogagalactnI8WN80|ibonaU|arrabiE53U6IYP6|aQn36CA07actaogalA0nib|aractanYB2ogalaniB6Vctanba6|arinogalaZba6rKa|D169SNP0A0A0galactan|arabinoC3RCI9alactanT2|arabinogA0A078RGaalC6YZ45|arabinogalactanE2NMA6|arabinogalactanR6KPQ5|arAinogalactan0A069D6Lab5|arabinogalactaU6RATnD7I1|arAa61C6IQ|arabinbinogalactaR9H23|arnogaR7K881|abinolactanQAK27arabigalact809AA|aranogaanY00R7Abinolacta38|a07QrAgalanab8Q8i1Rn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