实验室常用溶液配方

蛋白质常用buffer

实验室常用溶液配方

PBS(1×)(1L) 8.0g NaCl

0.2g KCl

1.44g Na2HPO4

(3.6283g Na2HPO4.12H2O

2.716g Na2HPO4.7H2O)

0.24g KH2PO4

80ml H2O

调至pH 7.4, add Milli-Q to 1L

Running Buffer(1×)(1L)

3g Tris

14.4g Gly

1g SDS

Add ddH2O to 1L

Transfer Buffer(1×)(1L)

5,8g Tris

2.9g Gly

0.37 SDS

200ml Methanol

Add ddH2O to 1L

TBE (5×)(1L)

54g Tris

27.5g Boric acid

20ml 0.5M EDTA (pH 8.0)

Add ddH2O to 1L

NP40 Lysis Buffer

5ml 3M NaCl

2.5ml 1M Tris-HCl (pH 6.8)

2.5ml 1M Tris-HCl (pH 8.0)

1ml NP40

Add ddH2O to 100ml

蛋白质常用buffer

LB Medium

5g NaCL

5g Yeast Extract

10g Tryptone

Add ddH2O to 1L

(Agar 2%)

1M Tris

用800ml ddH2O 溶解121.1g Tris, 加浓盐酸调pH至所需值。

pH HCl

7.4 70ml

7.6 60ml

8.0 42ml

EDTA(0.5M pH 8.0)

将186.1g EDTA-Na.2H2O加入800ml水中，在磁力搅拌器上剧烈搅拌。用NaOH调节溶液pH值至8.0（约需20gNaOH）,定容至1L。EDTA二钠盐需加入NaOH将溶液pH 值调至接近8.0时，才会溶解。

30%丙烯酰胺混合物（m/v）（100ml）

29.22g acrylamid

0.78g bisacrylamid

Add 100ml ddH2O

PEM Buffer

200ml 400Mm Potassium PIPES

(pH6.8)

10ml 0.5M EGTA (pH 7.0)

2ml 1.0M MgCl2

TBST Buffer(1×)(1L)

8.8g NaCl

0.2g KCl

3g Tris-base

500μl Tween-20

蛋白质常用buffer

组织裂解液：0.1M Tris-HCl (pH=8.5)

5mM EDTA (pH=8.0)

0.2% SDS

200mM NaCl

1L RIPA buffer: 50mM Tris-HCl (pH 7.4) 6.1g

1.0mM EDTA

150mM NaCl

0.1%SDS

1%NP40

0.25% sodium deoxyholate（脱氧胆酸钠）

4ml(0.25M) 8.8g 1g 10ml 2.5g

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