E.coli genetype 大肠杆菌基因型

大肠杆菌基因型和参考文献

Cloning & Expression

Genotypes of Selected E. coli Strains

Genotypes of Selected E. coli Strains

BL21 E. coli B F– ompT gal [E. coli B is naturally dcm and lon] hsdSB

BL21(DE3) E. coli B F– ompT gal [E. coli B is naturally dcm and lon] hsdSB with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIQ

C600 F– [e14– (McrF–) or e14+ (McrF+)] thr-1 leuB6 thi-1 lacY1 glnV44 rfbD1 fhuA21

CJ236 F Δ(HindIII)::cat (Tra+ Pil+ CamR )/ ung-1 relA1 dut-1 thi-1 spoT1 mcrA

GC5 F´ endA1 hsdR17 (rK–mK+) glnV44 thi-1 recA1 gyrA (Nalr) relA1 Δ(lacIZYA-argF)U169

(φ80dlacΔ(lacZ)M15 fhuA

GM48 F– thr leu thi lacY galK galT ara fhuA tsx dam dcm glnV44

HB101 F– Δ(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13

JM83 F– ara Δ(lac-proAB) rpsL (Strr)[φ80 dlacΔ(lacZ)M15] thi

JM101 F´traD36 proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi

JM103 F´ traD36 lacIqΔ(lacZ)M15 proA+B+/endA1 glnV sbcBC thi-1 rpsL (Strr) Δ(lac-pro) (P1) (rK–mK+ rP1+ mP1+)

JM105 F´ traD36 lacIqΔ(lacZ)M15 proA+B+/thi rpsL (Strr) endA sbcB15 sbcC hsdR4 (rK–mK+) Δ(lac-proAB)

JM107 F´ traD36 lacIq Δ(lacZ)M15 proA+B+/e14–(McrA–) Δ(lac-proAB) thi gyrA96 (Nalr) endA1 hsdR17 (rK– mK+) relA1 glnV44

JM109 F´traD36 proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV44 e14- gyrA96 recA1 relA1 endA1 thi hsdR17

JM110 F´ traD36 lacIqΔ(lacZ)M15 proA+B+IrpsL (Strr) thr leu thi lacY galK galT ara fhuA dam dcm glnV44 Δ(lac-proAB)

大肠杆菌基因型和参考文献

K802 F– e14- (McrA-) lacY1 or Δ(lac)6 glnV44 galK2 galT22 rfbD1 metB1 mcrB1 hsdR2 (rK–mK+)

LE392 F– e14– (McrA–) hsdR514 (rK–mK+) glnV44 supF58 lacY1 or Δ(lacIZY)6 galK2 galT22 metB1 trpR55

MC1061 F– araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (Strr) hsdR2 (rK–mK+) mcrA mcrB1

MM294 F– endA1 hsdR17 (rK–mK+) glnV44 thi-1 relA1 rfbD1 spoT1

NM477 C600 Δ(hsdMS-mcrB)5 (rK–mK+ McrBC–)

NM522 F´proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5

NM554 MC1061 recA13

NM621 F– hsdR (rK–mK+) mcrA mcrB glnV44 recD1009

RR1 HB101 RecA+

χ1776 F– fhuA53 dapD8 minA1 glnV44 Δ(gal-uvrB)40 minB2 rfb-2 gyrA25 (Nalr) thyA142 oms-2 metC65 oms-1 (tte-1) Δ(bioH-asd)29 cycB2 cycA1 hsdR2 (rK– mK+) mcrB1

References

1. New England Biolabs. E. coli Strain Genotypes.

/nebecomm/tech_reference/restriction_enzymes/ecoli_genotypes.asp

(Oct. 31, 2005)

2. Wertman, K.F. et al. (1986) Gene 49, 253–262.

3. Yanisch–Perron, C., Viera, J. and Messing, J. (1985) Gene 33, 103–119.

4. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory

Manual, (2nd ed.). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

5. Huynh,T.V. et al. (1985). In D.M. Glover (Ed.), DNA Cloning Vol. 1, (pp. 56–110). Oxford,

England: IRL Press Limited.

6. Raleigh, E.A. et al. (1988) Nucl. Acids Res. 16, 1563–1575.

7. Woodcock, D.M. et al. (1989) Nucl. Acids Res. 17, 3469–3478.

8. Raleigh, E.A., Lech, K. and Brent, R. (1989). In F.M. Ausebel et al. (Eds.), Current Protocols

in Molecular Biology (p. 1.4). New York: Publishing Associates and Wiley Interscience.

9. Berlyn, M.K.B. (1996). In F.C. Niedhardt et al. (Ed.), Escherichia coli and Salmonella:

cellular and molecular biology, (2nd ed.), Vol. 2, (pp. 1715–1902). ASM Press.

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10. Miller, J.H. (1972). Experiments in Molecular Genetics. Cold Spring Harbor: Cold Spring

Harbor Laboratory Press.

11. Whittaker, P.A. et al. (1989) Nucl. Acids Res. 16, 6725–6736.

12. Murray, N.E. et al. (1977) Mol. Gen. Genet. 150, 53–61.

13. Palmer, B.R. and Marinus, M.G. (1994) Gene 143, 1–12.

14. Boyer, H.W, and Roulland–Dussoix, D. (1969) J. Mol. Biol. 41, 459.

15. Silhavy, T.J. et al. (1984) Experiments with Gene Fusions (pp. xi–xii) Cold Spring Harbor:

Cold Spring Harbor Laboratory.

16. Bullock, W.O. et al. (1987) BioTechniques 5, 376–378.

17. Maurizi, M.R. et al. (1985) J. Bacteriol. 164, 1124–1135.

18. Studier, F.W. et al. (1990). In D.V. Goeddel (Ed.), Methods in Enzymology Vol. 185, (pp.

60–89). San Diego: Academic Press.

19. Kelleher, J. and Raleigh, E.A. (1991) J. Bacteriol. 173, 5220–5223.

20. Woodcock, D.M. et al. (1989) Nucl. Acids Res. 17, 3469–3478.

21. Palmer, B.R. and Marinus, M.G. (1994) Gene 143, 1–12.

22. Yanisch-Perron, C., Viera, J. and Messing, J. (1985) Gene 33, 103–119.

23. Messing, J. (1979) Recombinant DNA Technical Bulletin (NIH) 2, 43–48.

24. Gough, J. and Murray, N. (1983) J. Mol. Biol. 166, 1–19.

25. Baker, T.A. et al. (1984) Proc. Nat. Acad. Sci. USA 81, 6779–6783.

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27. Chung, C.H. and Goldberg, A.L. (1981) Proc. Nat. Acad. Sci. USA 78, 4931–4935.

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TB1 (E.Coli) Any Info Helpful

Michael Benedik

Fri Dec 4 02:29:53 EST 1992

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大肠杆菌基因型和参考文献

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In article <1992Dec3.182959.10559 at vax.oxford.ac.uk>, dnicker at vax.oxford.ac.uk writes:

>

> Am looking for genotype and important characteristics of E.Coli Strain

>TB1, ref.

> Sliger(sp?), S.G. et.al.

>m.,169,1016-1020 (1990)

>

> TB1 is mentioned in this paper, but no reference given, and all >attempts at identifying this strain have failed. Anyone heard of it? >

> Thanks in advance for any help you may provide. . .

>

> Darren Nickerson > D. Phil. Student > Univ. of Oxford >

>E-mail: DNICKER at VAX.OX.AC.UK

There is a standard strain TB1 used in a lot of molecular biology

labs. I don't know whether that is what you have or not becuase other people may have called their strain TB1 also. But the standard strain is an hsdR- derivative (restriction minus) of JM83.

genotype should be: F- ara del(lac-pro) rpsL phi80dlacZM15

in other words the relevent genotype for you is

blue white screen for cloning in pUC and derivatives, F- so no M13, Pro- so needs proline on minimal plates, restriction minus, strep resistant,

Some version of TB1 floating out there also have a Tn5 which was used to transduce in the hsdR allele, but the real strain is not supposed to have it. The precursor to TB1 accidentally got released for awhile.

---------------------------------------------------------------------- Michael Benedik INTERNET: Benedik at uh.edu

Dept. of Biochemical & Biophysical Sciences

University of Houston BITNET: Benedik at uhou

大肠杆菌基因型和参考文献

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