PCR设计引物时酶切位点的保护碱基表

PCR设计引物时酶切位点的保护碱基表1

PCR设计引物时酶切位点的保护碱基表2

Pme I

GTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG

0 0 0 75 0 10 >90 >90 0 0 10 0 10 0 50 0 10 10

0 25 50 >90 0 10 >90 >90 0 0 25 10 10 0 >90 0 50 75

Pst I

GCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT

Pvu I

CCGATCGG ATCGATCGAT TCGCGATCGCGA

Sac I Sac II

CGAGCTCG GCCGCGGC TCCCCGCGGGGA

Sal I

GTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCG G ACGCGTCGACGTCGGCCATAGCGGCCGCG GAA

Sca I

GAGTACTC AAAAGTACTTTT

10 75 0 0 10 >90 10 10 0 0 0 0 10 >90 >90 >90

25 75 10 10 50 >90 >90 >90 50 50 0 25 50 >90 >90 >90

Sma I

CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA

Spe I

GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG

Sph I

GGCATGCC CATGCATGCATG ACATGCATGCATGT

Stu I

AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT

Xba I

CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG

0 >90 75 75 0 10 10 0 25 50 >90

0 >90 >90 >90 0 25 75 0 75 >90 >90

Xho I

CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG

Xma I

CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA

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