在体短距离细小神经记录复合神经动作电位的最佳方法

在体周围神经记录复合神经动作电位的研究很少,尤其在体短距离细小神经记录复合神经动作电位尚未见有研究报道。中国复旦大学华山医院的劳杰教授与华山医院肌电图室合作多次探索进行预实验记录复合神经动作电位,不仅发现了记录的最佳方法,而且发现了小的不同电极之间的距离对复合神经动作电位参数有明显影响。

在体短距离细小神经记录复合神经动作电位的最佳方法

在体周围神经记录复合神经动作电位的研究很少，尤其在体短距离细小神经记录复合神经动作电位尚未见有研究报道。中国复旦大学华山医院的劳杰教授与华山医院肌电图室合作多次探索进行预实验记录复合神经动作电位，不仅发现了记录的最佳方法，而且发现了小的不同电极之间的距离对复合神经动作电位参数有明显影响。实验在完全游离大鼠正中神经后，难以记录到复合神经动作电位，但保留神经中部系膜，将地线置于系膜下，两端放置记录和刺激电极，即可恒定记录到复合神经动作电位。研究结果表明：在大鼠正中神经记录复合神经动作电位，记录电极间距离和刺激电极间距离均以5mm为最佳，刺激电极至记录电极间最佳距离为10mm，顺向记录优于逆向记录。该方法不仅可以为基础研究提供参考。也可为临床探索短的臂丛神经根部或干部记录复合神经动作电位提供新的研究思路。研究成果发表于《中国神经再生研究（英文版）》杂志2014年1月第2期。

记录复合神经动作电位的不同方法。（A）刺激电极(记录电极)的两针分别放在游离两端的正中神经下面,神经中断组织未游离，地线插入未游离的软组织内。（B）电极的两针分别放在游离两端的正中神经下面,神经完全游离，地线贴在神经中断下面。（C）电极的两针分别放在游离两端的正中神经下面,神经完全游离，地线插入神经下面的软组织内。Sti：刺激电极；Rec：记录电极；Gnd：地线

Article: " The optimal distance between two electrode tips during recording of compound nerve action potentials in the rat median nerve " by Yongping Li1, 2, Jie Lao1, Xin Zhao1, Dong Tian1, Yi Zhu1, Xiaochun Wei2 (1 Department of Hand Surgery of HuaShan Hospital, Fudan University; Key Laboratory of Hand Reconstruction, Ministry of Healthy; Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai, China; 2 Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China)

Li YP, Lao J, Zhao X, Tian D, Zhu Y, Wei XC. The optimal distance between two electrode tips during recording of compound nerve action potentials in the rat median nerve. Neural Regen Res. 2014;9(2):171-178.

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Optimal distance between two electrode tips during recording of compound nerve action potentials

在体周围神经记录复合神经动作电位的研究很少,尤其在体短距离细小神经记录复合神经动作电位尚未见有研究报道。中国复旦大学华山医院的劳杰教授与华山医院肌电图室合作多次探索进行预实验记录复合神经动作电位,不仅发现了记录的最佳方法,而且发现了小的不同电极之间的距离对复合神经动作电位参数有明显影响。

The distance between the two electrode tips can greatly infuence the parameters used for recording compound nerve action potentials. To investigate the optimal parameters for these recordings in the rat median nerve, Prof. Jie Lao and colleagues from HuaShan Hospital, Fudan University in China dissociated the nerve using different methods and compound nerve action potentials were orthodromically or antidromically recorded with different electrode spacings. Compound nerve action potentials could be consistently recorded using a method in which the middle part of the median nerve was intact, with both ends dissociated from the surrounding fascia and a ground wire inserted into the muscle close to the intact part. A distance of 5 mm between recording and stimulating electrodes and a distance of 10 mm between recording and stimulating sites were found to be optimal for compound nerve action potential recording in the rat median nerve. In addition, the

orthodromic compound action potential, with a biphasic waveform that was more stable and displayed less interference (however also required a higher threshold and higher supramaximal stimulus), was found to be superior to the antidromic compound action potential.

The different approaches to compound nerve action potential recording.

Both ends of the median nerve had electrodes placed, leaving the middle part of approximately 4–5 mm intact. The two electrodes were placed under both ends of the nerve trunk, distal and proximal to the middle. The ground wire was inserted into the muscle close to the intact part (Method A; n = 10). In Method B (n = 10), the median nerve was completely dissociated, then otherwise treated as in Method A. In the third approach, the ground wire was placed beneath the middle part of the completely dissociated median nerve trunk (Method C; n = 10). Sti: Stimulating electrodes; Rec: recording electrodes; Gnd: ground wire.

Article: " The optimal distance between two electrode tips during recording of compound nerve action potentials in the rat median nerve " by Yongping Li1, 2, Jie Lao1, Xin Zhao1, Dong Tian1, Yi Zhu1, Xiaochun Wei2 (1 Department of Hand Surgery of HuaShan Hospital, Fudan University; Key Laboratory of Hand Reconstruction, Ministry of Healthy; Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai, China; 2 Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China)

Li YP, Lao J, Zhao X, Tian D, Zhu Y, Wei XC. The optimal distance between two electrode tips during recording of compound nerve action potentials in the rat median nerve. Neural Regen Res. 2014;9(2):171-178.

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